产品货号：
Z14159
中文名称：
Cofilin/CFL1抗体
英文名称：
Anti-Cofilin/CFL1 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-Cofilin/CFL1 Antibody |
| 指标别名 | 18kDa phosphoprotein;CFL;CFL1;Cofilin 1;cofilin 1 (non muscle);Cofilin;non muscle isoform;p18 |
| 克隆性 | Polyclonal |
| 检验物种 | human,monkey,mouse,rat |
| 应用范围 | WB,IHC,IHC-F,ICC/IF,FCM,ELISA |
| 基因名称 | CFL1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human Cofilin recombinant protein (Position:A2-L166).Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin. |
| 实际分子量 | 19KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Cofilin,a member of the ADF/cofilin family,is actually a protein with 70% sequence homology to ADF,making it part of the ADF/cofilin family of small ADP-binding proteins.It is a ubiquitous actin-binding factor required for the reorganization of actin filaments.The protein is known to sever actin filaments by creating more positive ends on filament fragments,it can cause depolymerization at the minus end of filaments,thereby preventing their reassembly.As a long-lasting in vivo effect,cofilin recycles older ADP-F-actin,helping cell to maintain ATP-G-actin pool for sustained motility.pH,phosphorylation and phosphoinositides regulate cofilin’s binding and associating activity with actin. |
| Uniprot ID | P23528 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | cofilin 1 (non-muscle) |
| 蛋白名全称 | Cofilin-1 |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunohistochemistry in frozen section (IHC-F) 1:50～400 Immunocytochemistry/Immunofluorescence(ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells ELISA 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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